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Invitrogen™ Dynabeads™ FlowComp™ Mouse CD8 Kit 
This gentle and easy-to-use kit allows you to isolate flow-compatible mouse CD8+ T cells. Immediately after positive isolation, the beads are released and removed from your cell sample.
Supplier: Invitrogen™ 11462D
Includes: 1mL Antibody Mix, 3mL FlowComp; Dynabeads and 40mL FlowComp; Release Buffer
Description
This gentle and easy-to-use kit allows you to isolate flow-compatible mouse CD8+ T cells. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.
You can expect a purity level of >85% and viability of >85% of mouse CD8+ cells.
Advantages
• No risk of your results being affected by the isolation method
• Gentle tube-based method—no columns needed
• High purity, recovery and viability
• Bead-free cells—compatible with flow cytometry analysis
• Viable, bead-free CD8+ T-cells for direct use in any cell-based assay.
The isolated cells have the presumed profile of memory and naive mouse CD8+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.
Starting sample
Spleen or lymph node cells
Order Info
Shipping Condition: Room Temperature
Specifications
| T Cells (CD8) | |
| Superparamagnetic Beads | |
| Processes ∼2 x 109 cells total | |
| DYNAL, Dynabeads, FlowComp | |
| 1 kit | |
| Lymph Node, Blood, Spleen | |
| 5 x 107 cells per isolation |
| The Kit Contains: • 1 mL Antibody Mix • 3 mL FlowComp™ Dynabeads™ • 40 mL FlowComp™ Release Buffer. Store at: 2°C to 8°C. |
|
| Positive Isolation | |
| >95% | |
| Research Grade | |
| Mouse | |
| Room Temperature | |
| Mouse |
For Research Use Only. Not for use in diagnostic procedures.