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Invitrogen™ CELLection™ Epithelial Enrich Dynabeads™ 
Use this kit to obtain up to 5-log enrichment of human epithelial tumor cells, directly from whole blood, bone marrow or PBMC.
Supplier: Invitrogen™ 16203
Includes: 5mL CELLection; Epithelial Enrich Dynabeads coated with a monoclonal antibody towards the human Epithelial Cell Adhesion Molecule (EpCam). 2 vials Releasing Buffer Component 1 (DNase I) and 2mL Relasing Buffer Component 2 are also supplied. Up to 2x109 cells can be processed with this kit.
Description
Use this kit to obtain up to 5-log enrichment of human epithelial tumor cells, directly from whole blood, bone marrow or PBMC. Tumor cell clumps can be enriched from bone marrow. Enriched cells are bead-free, viable and suitable for immunocytochemical staining or any other downstream application.
High enrichment of viable human tumor cells
• Isolate directly from whole blood, bone marrow or PBMC
• Viable cells for any cellular applications
Methodology: Add CELLection™ Dynabeads™ to the sample. The beads will bind the tumor cells after a short incubation. The bead-bound tumor cells are quickly separated on a magnet and subsequently released from the beads with the supplied Release Buffer. (CELLection™ Dynabeads™ contains a DNA linker that is cleaved by the DNase I in the Release Buffer.)
Note: If you want to deplete or use the isolated tumor cells for molecular applications, please use the equivalent product Dynabeads™ Epithelial Enrich (Cat. No. 161-02). This product contains the same antibody, but with no release of the cells from the beads after positive isolation.
Starting Samples: Whole blood, bone marrow, buffy coat, PBMC.
Order Info
Shipping Condition: Room Temperature
Specifications
| Tumor Cells, Epithelial Cells | |
| Epithelial Enrichment Kit | |
| Processes ∼4 x 109 cells total | |
| CELLection, DYNAL, Dynabeads | |
| 5 mL | |
| PBMC, Blood | |
| 2 x 107 PBMCs per isolation |
| Each Kit Contains: • 5 mL CELLection™ Epithelial Enrich Dynabeads™ coated with a monoclonal antibody towards the human Epithelial Cell Adhesion Molecule (EpCam) • 2 vials Releasing Buffer Component 1 (DNase I) • 2 mL Releasing Buffer Component 2 Up to 2 x 109 cells can be processed with this kit. Store components at 2°C to 8°C. |
|
| Positive Isolation | |
| >95% | |
| Research Grade | |
| Human | |
| Room Temperature | |
| Human |
For Research Use Only. Not for use in diagnostic procedures.