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Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment DFS Item

DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities

$144.90 - $548.10

Specifications

Product Type Polymerase (1), DNA
For Use With (Application) Array CGH, Cloning, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Restriction Enzyme Cloning
Includes DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact 2 Buffer [500mM Tris-HCl (pH 8.0), 100mMmgCl2, 500mM NaCl], vial of dilution buffer
Quantity 500 units
View More Specs
Includes:  DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500mM Tris-HCl (pH 8.0), 100mM MgCl2, 500mM NaCl], vial of dilution buffer.

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

18012039

DFS Item
 
life technologies
18012039
500 units Each for $548.10

18012021

DFS Item
 
life technologies
18012021
100 units Each for $144.90
Description & Specifications

Specifications

Product Type Polymerase (1), DNA
For Use With (Application) Array CGH, Cloning, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Restriction Enzyme Cloning
Includes DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact 2 Buffer [500mM Tris-HCl (pH 8.0), 100mMmgCl2, 500mM NaCl], vial of dilution buffer
Quantity 500 units

Applications:
Fill-in of 5' overhangs . Synthesis of probes by random primers labeling method. Sequencing single- and double-stranded DNA. Site-directed mutagenesis.

Source:
Purified from E. coli expressing the Klenow fragment on a plasmid.

Performance and Quality Testing:
SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.

Unit Definition:
One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer.

Array CGH, Cloning, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Restriction Enzyme Cloning

Shipping Condition: Approved for shipment on Wet or Dry Ice