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Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment DFS Item

DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities

$149.00 - $564.00

Specifications

Product Type Polymerase (1), DNA
For Use With (Application) Array CGH, Cloning, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Restriction Enzyme Cloning
Includes DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact 2 Buffer [500mM Tris-HCl (pH 8.0), 100mMmgCl2, 500mM NaCl], vial of dilution buffer
Quantity 100 units
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes: DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500mM Tris-HCl (pH 8.0), 100mM MgCl2, 500mM NaCl], vial of dilution buffer.


Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
18012039 Life Technologies
LS18012039
500 units Each for $564.00
18012021 Life Technologies
LS18012021
100 units Each for $149.00
Specifications

Description

Applications:
Fill-in of 5' overhangs . Synthesis of probes by random primers labeling method. Sequencing single- and double-stranded DNA. Site-directed mutagenesis.

Source:
Purified from E. coli expressing the Klenow fragment on a plasmid.

Performance and Quality Testing:
SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.

Unit Definition:
One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer.

Array CGH, Cloning, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Restriction Enzyme Cloning

Order Info

Shipping Condition: Approved for shipment on Wet or Dry Ice

Description & Specifications

Specifications

Polymerase (1), DNA
Array CGH, Cloning, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Restriction Enzyme Cloning
DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact 2 Buffer [500mM Tris-HCl (pH 8.0), 100mMmgCl2, 500mM NaCl], vial of dilution buffer
100 units