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  6. 18162016

Invitrogen™ DNA Polymerase I/DNase I

Catalog No. 18162016
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250 U
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Catalog No. Quantity
18162016 250 U
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Catalog No. 18162016 Supplier Invitrogen™ Supplier No. 18162016
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Description

Includes

DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter.

An optimized mixture of both enzymes for efficient nick translation of DNA

  • Application: Labeling DNA with either radiolabeled or biotinylated nucleotides
  • Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen nM 964
  • Performance and Quality Testing: Performance tested in nick translation reaction
  • Unit Definition: One unit of DNA Polymerase I in the absence of DNase I incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template primer
  • Unit Reaction Conditions: 50mM potassium phosphate (pH 7.5), 6.7mM MgCl2 , 1mM 2-mercaptoethanol, 80μg/mL template•primer, 32μM dTTP, 69nM [3H]dTTP, and enzyme in 100μL for 30 min. at 37°C

Cloning, Restriction Enzyme Cloning

Order Info

Shipping Condition: Wet Ice

Specifications

Content And Storage DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter. Store at -20°C.
Description DNA Polymerase
Polymerase DNA Polymerase I
Exonuclease Activity 3' - 5', 5' - 3'
Quantity 250 U
Shipping Condition Wet Ice
Hot Start No

For Research Use Only. Not for use in diagnostic procedures.