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Invitrogen™ DNA Polymerase I DFS Item

DNA polymerase with 5'→3' and 3'→5' exodeoxyribonuclease activities

$294.00 - $928.20

Specifications

Product Type Polymerase (1), DNA
For Use With (Application) Cloning, Restriction Enzyme Cloning
Includes 1000 units of DNA polymerase in a storage buffer of 50mM potassium phosphate (pH 7.0), 100mM KCl, 1mM DTT, and 50% (v/v) glycerol. It is supplied at 5–10 units/μL
Quantity 250 units
View More Specs
Includes:  1000 units of DNA polymerase in a storage buffer of 50mM potassium phosphate (pH 7.0), 100mM KCl, 1mM DTT, and 50% (v/v) glycerol. It is supplied at 5-10 units/μL.

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

18010017

DFS Item
 
life technologies
18010017
250 units Each for $294.00

18010025

DFS Item
 
life technologies
18010025
1000 units Each for $928.20
Description & Specifications

Specifications

Product Type Polymerase (1), DNA
For Use With (Application) Cloning, Restriction Enzyme Cloning
Includes 1000 units of DNA polymerase in a storage buffer of 50mM potassium phosphate (pH 7.0), 100mM KCl, 1mM DTT, and 50% (v/v) glycerol. It is supplied at 5–10 units/μL
Quantity 250 units

Performance and quality testing
Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.

Unit definition

One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.

Unit reaction conditions

50mM potassium phosphate (pH 7.0), 6.7mM MgCl2 , 1mM 2-mercaptoethanol, 80μg/mL template/primer, 32μM dTTP, 69nM [3H]dTTP, and enzyme in 100μL for 30 min at 37°C. Also incorporates biotinylated nucleotides.

  • Applications: DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5' overhangs
  • Source: purified from E. coli expressing the DNA Polymerase I gene on a plasmid

Cloning, Restriction Enzyme Cloning

Shipping Condition: Wet Ice