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Invitrogen™ 5-(3-Aminoallyl)-dUTP (50mM) DFS Item

When incorporated into DNA, 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups

Manufacturer:  Invitrogen™ LSAM8439

Catalog No. AM8439



Description

Description

The modified dUTP is readily incorporated by reverse transcriptase. The aminoallyl modification enables downstream reaction with amine-reactive compounds such as N-hydroxysuccinimide (NHS) esters; thus aminoallyl-modified cDNA can be labeled with any amine-reactive group moiety.

  • Use of this Modified Nucleotide: Use 5-(3-aminoallyl)-dUTP as recommended in your protocol for reverse transcription
  • Substitution of 30–80% of the dTTP with 5-(3-aminoallyl)-dUTP may be useful, but the exact proportion will depend on experimental goals

Gene Expression Analysis & Genotyping, in vitro Transcription

Order Info

Shipping Conditions: Dry Ice

Specifications

Specifications

50mM
Modified UTPs
50μL
Store at or below -70°C. Avoid multiple freeze-thaw cycles. Aliquots of the product may be stored short-term at -20°C. Do not store in a frost-free freezer.
Documents

For Research Use Only. Not for use in diagnostic procedures.