Invitrogen™ 5-(3-Aminoallyl)-dUTP (50 mM)

Lorsqu’elle est incorporée à l’ADN, la Ambion 5-(3-aminoallyl)-dUTP fournit un groupe réactif pour l’ajout d’autres groupes chimiques.

When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups. The modified dUTP is readily incorporated by reverse transcriptase. The aminoallyl modification enables downstream reaction with amine-reactive compounds such as N-hydroxysuccinimide (NHS) esters; thus aminoallyl-modified cDNA can be labeled with any amine-reactive group moiety.

Use of this modified nucleotide
Use 5-(3-aminoallyl)-dUTP as recommended in your protocol for reverse transcription. Substitution of 30–80% of the dTTP with 5-(3-aminoallyl)-dUTP may be useful, but the exact proportion will depend on experimental goals.

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