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Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer Set

Numéro de catalogue. 501129037
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501129037 1 kit
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Contains Sodium azide and formaldehyde
Numéro de catalogue. 501129037 Fournisseur Invitrogen™ Code fournisseur 88882400
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Contains Sodium azide and formaldehyde

Description

Comprend

  • IC Fixation Buffer
  • Permeabilization Buffer (10X)

Designed for use in intracellular staining and flow cytometric analysis and has been specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and increase fluorescence signal-to-noise ratios.

  • In the first step, live cells can be 'fixed' with the Fixation Buffer, which cross- links proteins
  • The second step, using the Permeabilization Buffer, creates holes in the membrane and allows the intracellular staining antibodies to enter the cell effectively
  • Subsequent washing steps, antibody additions, and incubations after cell permeabilization should be performed using the Permeabilization Buffer
  • Final washing step and cell resuspension prior to running the sample is done using Flow Cytometry Staining Buffer (cat. 00-4222).
  • This kit is commonly used to stain cytoplasmic proteins and proteins in the secretory pathway such as chemokines and cytokines
  • Not recommended for intranuclear staining. Instead, please refer to the Foxp3/Transcription Factor Buffer Set for nuclear staining
  • Before the fixation step, it is recommended to label the live cells using one of the Fixable Viability Dyes and then proceed with fixation

Spécifications

Product Line eBioscience
Product Type Fixn/Perm Buffer Set
Quantity 1 kit
Solution Type Fixation/Permeabilization Buffer

For Research Use Only. Not for use in diagnostic procedures.