Recombinant Protein;Biotinylated Human IL-4 Protein, Avi Tag (Avitag™);200UG;Biotinylated Human IL-4, His Tag (IL4-H82E0) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # AAH67514).;This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 17.6 kDa. As a result of glycosylation, the protein migrates as 22 kDa under reducing (R) condition, and 21 kDa under non-reducing (NR) condition (SDS-PAGE).;Immobilized Human IL-4 R alpha, His Tag (Cat. No. ILR-H5221) at 5 g/mL (100 L/well) can bind Biotinylated Human IL-4, His Tag (Cat. No. IL4-H82E0) with a linear range of 1.95-15.6 ng/mL (QC tested).;Biotinylated Human IL-4, His Tag, Avi Tag (Cat. No. IL4-H82E0) stimulates the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1.19-2.64 ng/mL (Routinely tested).