Invitrogen™ Silencer™ Select Negative Control No. 1 siRNA

Negative controls, such as non-targeting siRNAs, are critical for distinguishing the specific effects of siRNA-mediated gene silencing from non-specific effects caused by the experimental procedure itself. Silencer Select negative control No. 1 is designed to ensure minimal off-target effects, providing a robust baseline for comparing the results of siRNA-treated samples. This negative control siRNA shares the same chemical modifications as other Silencer Select siRNAs, ensuring enhanced efficacy and specificity.

Properties of Silencer Select Negative Control siRNAs include:

• No significant sequence similarity to human mouse, or rat genes
• Minimal effects on cell proliferation and viability
• Silencer Select modifications for enhanced specificity

Validated through extensive testing, including microarray analysis and multi-parametric cell-based assays, Silencer Select Negative Control No. 1 has been proven to have minimal impact on gene expression, cell proliferation, viability, and morphology. This makes it an essential tool for researchers aiming to achieve accurate and consistent phenotypic data in human, mouse, and rat cells.

What are Silencer Select siRNAs?

Silencer Select siRNAs represent our next-generation siRNAs, incorporating the latest advancements in siRNA design, off-target effect prediction algorithms, and chemical modifications. These improvements result in siRNAs with unrivalled efficacy, potency, and specificity, leading to fewer failed experiments and more consistent, cleaner phenotypic data.

Quality control

Each Silencer Select siRNA is synthesized and purified in state-of-the-art facilities to meet the highest quality standards. Our rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. We also assess each annealed siRNA by gel electrophoresis to ensure proper strand annealing. The result is a premium-quality siRNA that is purified and ready for use.

Lipofectamine™ RNAiMAX™ Transfection Reagent (SKU 13778075)

Lipofectamine™ RNAiMAX™ Transfection Reagent is a leading transfection reagent designed specifically for RNA interference applications. The protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications. Silencer Select siRNA controls have specific chemical modifications that are designed to be used with Silencer Select siRNAs. If you are using other siRNA products, such as Silencer siRNA or Stealth RNAi siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.