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Invitrogen™ Pichia Expression Kit, original kit

Catalog No. K171001
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Catalog No. K171001 Supplier Invitrogen™ Supplier No. K171001
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Description

The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris.

The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris. The vectors carry the HIS4 gene for selection of transformants on histidine-deficient medium. The Original Pichia Expression Kit provides all of the tools and reagents needed to express a gene of interest from the AOX1 promoter.

Specifications

Antibiotic Resistance Bacterial Ampicillin (AmpR)
Bacterial or Yeast Strain GS115, KM71
Content And Storage The Original Pichia Expression Kit includes 10 μg each of pHIL-D2 and pPIC3.5 vectors for intracellular expression and pHIL-S1 and pPIC9 vectors for secreted expression, Pichia pastoris strains GS115 (his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, media (YP and YNB), Spheroplast Transformation Kit, and 2 μg each 5' AOX1, 3' AOX1, and α-factor sequencing primers.

Store vectors and primers at -20°C. Store strains at room temperature.
All components are guaranteed stable for 6 months when properly stored.
Cloning Method Restriction Enzyme⁄ MCS
Promoter AOX1
Cell Type Yeast Cells
Format Kit
Species P. pastoris
Vector pPIC
Selection Agent (Eukaryotic) Ampillicin
Expression Mechanism Cell-Based Expression
Expression System Yeast
For Use With (Application) Protein Expression
Product Type Expression Kit
Protein Tag Untagged
Quantity 1 Kit
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For Research Use Only. Not for use in diagnostic procedures.