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  6. Gibco™ Human Skeletal Myoblasts (HSkM)

Gibco™ Human Skeletal Myoblasts (HSkM)

Catalog No. p-7075967
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1 vial x 5x106 cells
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A11440 1 vial x 5x106 cells
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Catalog No. A11440 Supplier Gibco™ Supplier No. A11440
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Description

Human Skeletal Myoblasts are developed to undergo highly efficient differentiation directly following plating of cryopreserved cells. Performance tested and guaranteed to differentiate ≥50% following 48 hours of incubation. Cells guaranteed to be ≥70% viable.

Primary normal human skeletal myoblasts (HSkM) are designed to undergo highly efficient differentiation directly following plating of cryopreserved cells. Each vial contains cells that have been cryopreserved in a medium containing 10% DMSO and will differentiate within 48 hours after plating. These cells do not require expansion prior to differentiation. Cell viability is >70% post thaw with a ≥50% myogenic index after 48 hours. They are intended for use by researchers investigating the molecular and biochemical bases of various normal and disease processes. They are compatible with Bacmam delivery technologies for various downstream applications. Large size contains 4.5 x 10^6–7.5 x 10^6 cells per vial and small size contains 1.1 x 10^6–1.9 x 10^6 cells per vial.

The cells are:

  • Performance tested—guaranteed to differentiate ≥50% following 48 hours of incubation
  • Guaranteed to be ≥70% viable
  • Tested for mycoplasma, bacteria, yeast or other fungi, hepatitis B, hepatitis C, and HIV-1 viruses

Each vial contain sufficient number of cells to fully seed a single multi-well dish (ranging in format from 6-well to 384-well).

Specifications

Content And Storage Store in liquid nitrogen.
Age Adult
Cell Type Skeletal Muscle Cells
Culture Type Adherent Cell Culture
Form Cryopreserved
Gender Mixed Gender
Species Human
Donor Attributes Normal
Donor Source Single Donor
Format Vial
No. of Cells 5 x 10^6
Product Line Gibco
Product Type Human Skeletal Myoblast
Quantity 1 vial x 5x106 cells
Tested For Mycoplasma, Bacteria, Yeast or Other Fungi, Hepatitis B, Hepatitis C, HIV-1 Virus
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Frequently Asked Questions (FAQs)

Have you performed any studies using the human skeletal myoblasts to replicate physiological conditions?

Please see the images below showing Gibco Human Skeletal Myoblasts responding to physiological levels of TGF-beta1 and IGF-1. TGF-beta1 addition inhibits differentiation, whereas the addition of IGF-1 (Cat. No. PHG0075) blunts this effect. The anti-troponin staining method was used.
What media do you use during the first passage of primary cells?

We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).
How is the population doubling calculated for primary cells?

Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.

What are typical primary cell yields from tissue?

It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.

Are primary cells isolated from tissue using enzymatic digestion?

Yes, our primary cells are isolated from tissue using enzymatic digestion.

What are primary cells?

Primary cells are cells taken directly from living tissue (e.g., biopsy material) and established for growth in vitro. These cells have undergone very few population doublings and are therefore more representative of the main functional component of the tissue from which they are derived in comparison to continuous (tumor or artificially immortalized) cell lines, making primary cells a more representative model for the in vivo state.

Primary cells from different species may be used, allowing you to highlight potential differences between humans and preclinical test species. Before in vivo studies, mouse or rat cells can be used to refine doses and reduce the number of animals required for preclinical toxicology. Human cells can be used to determine the accuracy of extrapolating human data from an animal model.

For Research Use Only. Not for use in diagnostic procedures.