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MilliporeSigma™ Human Dopaminergic Neurogenesis Kit

Catalog No. SCR135
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225 mL Media and Growth Factor Kit
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Catalog No. Quantity
SCR135 225 mL Media and Growth Factor Kit
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Catalog No. SCR135 Supplier MilliporeSigma™ Supplier No. SCR135
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Description

Contains necessary growth factors and complete differentiation media needed to differentiate human ES/iPS cells into dopaminergic neurons using 21 day differentiation protocol. MilliporeSigma™; Human Dopaminergic Neurogenesis Kit has been validated with 4 human ES and iPS lines for efficient dopaminergic neuronal differentiation.

  • Contains collection of five validated growth factors that are routinely used to induce differentiation of human pluripotent ES/iPS cells to dopaminergic neurons 
  • Has high affinity antibody marker, anti-tyrosine hydroxylase, to aid in quantifying percentage yields from differentiation experiments
  • H1: 30% TH positive neurons
  • H9: 50-70% TH positive neurons
  • Two Human iPS cell lines: ∼ 50% TH positive neurons
  • Components:
  • 2 vials of human recombinant Sonic Hedgehog (Shh) (5ug/vial)
  • 1 vial of human recombinant FGF-8 (5ug/vial)
  • 1 vial of human recombinant BDNF (2ug/vial)
  • 1 vial of human recombinant GDNF (2ug/vial)
  • 1 vial of human recombinant TGF-b-III (2ug/vial)
  • 1 vial of anti-tyrosine hydroxylase, rabbit polyclonal (20uL)

Specifications

Product Type Neurogenesis Kit
For Use With (Application) Cell Differentiation, Stem Cell Culture, Cell Culture, Neurotoxicity, Neuroscience
Quantity 225 mL Media and Growth Factor Kit
Regulatory Status RUO
Content And Storage
  • Human ES/iPS Neural Induction Medium: (Catalog No. SCM110)
  • Human ES/iPS Neuronal Differentiation Medium: (Catalog No. SCM111)
  • Dopaminergic Differentiation Growth Factor Sampler (Cat. No. SCR128)
All components within SCR135 (Cat. No. SCM110, SCM111 and SCR128) should be stored at -20°C upon receiving.