Recombinant Protein;100UG;Human CD96, Mouse IgG2a Fc Tag, low endotoxin (TAE-H5252) is expressed from human 293 cells (HEK293). It contains AA Val 22 - Met 503 (Accession # P40200-2).;This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 80.4 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human CD96, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. TAE-H5252) at 2 g/mL (100 L/well) can bind Human CD155, Fc Tag (Cat. No. CD5-H5251) with a linear range of 20-312 ng/mL (QC tested).;FACS analysis of Human CD96, Mouse IgG2a Fc Tag binding to CD155 cells overexpressing CD155.
2e5 of CD155 cells overexpressing CD155 were stained with 100 L of 1 g/mL of Human CD96, Mouse IgG2a Fc Tag (Cat. No. TAE-H5252) and negative control protein respectively, washed and then followed by PE-anti-Mouse IgG2a antibody and analyzed with FACS (Routinely tested).
