Recombinant Protein;1MG;Human HMGB1, His Tag (HM1-H5220) is expressed from human 293 cells (HEK293). It contains AA Gly 2 - Glu 215 (Accession # NP_002119.1).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.0 kDa. The protein migrates as 32 kDa and 35 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.;Human HMGB1, His Tag (Cat. No. HM1-H5220) stimulates production of TNF- in Raw-246.7 cells. The EC50 for this effect is 0.7855-0.8342 g/mL (Routinely tested).
