Cytiva HiTrap™ Capto™ IEX Selection Kit

Description

• Consists of five HiTrap columns, prepacked with Capto ion exchange chromatography media with different characteristics
• For media screening; includes five 1mL HiTrap columns prepacked with Capto Q, Capto S, Capto DEAE, Capto MMC, Capto adhere, as well as connectors and instructions
• For optimization studies; buffer composition, pH, flow rate, sample loading and elution schemes can be optimized with small sample quantities prior to scale-up
• Capto Q is strong anion exchanger, Capto DEAE is weak anion exchanger, Capto S is strong cation exchanger
• Capto MMC and Capto adhere media have multimodal characteristics that can be used to solve specific purification problems
• Capto MMC is multimodal cation exchanger that is functional at high as well as at low ionic strength
• Capto adhere is multimodal strong anion exchanger mainly designed for post-protein A purification of monoclonal antibodies
• Prepacked columns and bulk media are available for larger scale preparative purifications

• Max. pressure (over packed bed during operation): 3 bar (42psi)
• Flow rate: <4mL/min.
• Complete packsize: 5 x 1mL
• Bed dimensions: 7 × 25mm
• Bed volume: 1mL

• pH Stability cleaning: 2 to 14
• Matrix: highly cross-linked agarose with dextran surface extender
• Average particle size: 90μm (d50V)
• pH Stability working range: 2 to 12
• Pressure/Flow specification: 0.3MPa at 700cm/hr., 1m diameter column, 20cm bed height

• Column I.D.: 7mm
• Material (column hardware): polypropylene (PP)