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Gibco™ HepExtend™ Media Kit DFS Item

HepExtend Media Kit

Supplier:  Gibco™ A2933701

Catalog No. A2933701


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Description

Description

The HepExtend™ Media Kit contains the new HepExtend Supplement (50X), as well as William's E Medium and Primary Hepatocyte Maintenance Supplement, providing the three components needed to optimize long term hepatocyte culture. HepExtend Supplement has been optimized for use with cryopreserved primary hepatocytes to improve cell viability, function and number of days in culture. This enables researchers to perform metabolic and toxicity experiments not achievable using standard culture conditions. Simply add the HepExtend Supplement and Primary Hepatocyte Maintenance Supplement to the William’s E Medium for a complete solution. The HepExtend supplement does not contain any small molecules or fetal bovine sera, components that are known to interfere with primary hepatocyte function.

  • Enabling—offers maximum cell viability and cell health when culturing primary plateable hepatocytes
  • Extended cell survival—days in culture can be extended to >10 days while maintaining normal cell morphology and bile canaliculi
  • Consistent—manufactured in a cGMP compliant facility using high quality materials

    Maintain P450 activity
    The HepExtend Media Kit can be used to help ensure metabolically competent hepatocytes for at least 10 days for use in downstream ADME/Tox applications not achieved with standard 5-day culture systems.

    Enhanced gene expression
    Using the components in the HepExtend Media Kit to culture primary human hepatocytes increases the basal gene expression of many key genes involved in drug metabolism and transport.

    Retain inducibility
    Nuclear receptor activation and gene inducibility are retained in cultures treated with HepExtend supplemented media.
    • Specifications

    Specifications

    Mammalian Cells
    Cell Culture Medium
    1 Kit
    • 1 William's E Medium (A1217601)
    • 1 Primary Hepatocyte Maintenance Supplements (CM4000)
    • 1 HepExtend™ Supplement (50X) (A2737501)

    Store medium at 2–8°C degrees and supplements at -5 to -30°C.
    Cryopreserved
    HepExtend
    Dry Ice
    Mammalian Cell Culture
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    For Research Use Only. Not for use in diagnostic procedures.