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Gibco™ HepExtend™ Media Kit

Catalog No. A2933701
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Description

HepExtend Media Kit

The HepExtend™ Media Kit contains the new HepExtend Supplement (50X), as well as William's E Medium and Primary Hepatocyte Maintenance Supplement, providing the three components needed to optimize long term hepatocyte culture. HepExtend Supplement has been optimized for use with cryopreserved primary hepatocytes to improve cell viability, function and number of days in culture. This enables researchers to perform metabolic and toxicity experiments not achievable using standard culture conditions. Simply add the HepExtend Supplement and Primary Hepatocyte Maintenance Supplement to the William’s E Medium for a complete solution. The HepExtend supplement does not contain any small molecules or fetal bovine sera, components that are known to interfere with primary hepatocyte function.

  • Enabling—offers maximum cell viability and cell health when culturing primary plateable hepatocytes
  • Extended cell survival—days in culture can be extended to >10 days while maintaining normal cell morphology and bile canaliculi
  • Consistent—manufactured in a cGMP compliant facility using high quality materials

    Maintain P450 activity
    The HepExtend Media Kit can be used to help ensure metabolically competent hepatocytes for at least 10 days for use in downstream ADME/Tox applications not achieved with standard 5-day culture systems.

    Enhanced gene expression
    Using the components in the HepExtend Media Kit to culture primary human hepatocytes increases the basal gene expression of many key genes involved in drug metabolism and transport.

    Retain inducibility
    Nuclear receptor activation and gene inducibility are retained in cultures treated with HepExtend supplemented media.

    • Specifications

    Cell Type Mammalian Cells
    Form Cryopreserved
    Product Type Cell Culture Medium
    Product Line HepExtend
    Quantity 1 Kit
    Shipping Condition Dry Ice
    Content And Storage • 1 William's E Medium (A1217601)
    • 1 Primary Hepatocyte Maintenance Supplements (CM4000)
    • 1 HepExtend™ Supplement (50X) (A2737501)

    Store medium at 2–8°C degrees and supplements at -5 to -30°C.
    Culture Type Mammalian Cell Culture

    Frequently Asked Questions (FAQs)

    What is HepExtend Supplement?

    HepExtend Supplement (50X) is an enriched supplement designed to enhance the culture lifespan (cell viability, function, and number of days in culture) of cryopreserved primary hepatocytes. We recommend using the supplement in conjunction with William's E Medium (Cat. No. A1217601) and Hepatocyte Maintenance Supplement Pack (Cat. No. CM4000), following the recommended hepatocyte culture methods. When cultured in the presence of the HepExtend Supplement, primary human hepatocyte lots will live and be functional for 10 days or longer depending on the lot of cells and other assay conditions. HepExtend Supplement does not contain growth factors, cytokines, fetal bovine serum, or pharmaceutical small molecules that are known to interfere with primary hepatocyte function.
    What are the components of the HepExtend Media Kit (Cat. No.A2933701)?

    The HepExtend Media Kit contains the following components needed to make the complete medium for hepatocyte culture:

    - HepExtend Supplement (50X) (Cat. No.A2737501)
    - Williams E Medium (500 mL) (Cat. No.A1217601)
    - Primary Hepatocyte Maintenance Supplements (Cat. No.CM4000)

    For Research Use Only. Not for use in diagnostic procedures.