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Gibco™ Hepatic Spheroid Kit

Catalog No. A41390
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Catalog No. A41390 Supplier Gibco™ Supplier No. A41390
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Description

The Gibco Hepatic Spheroid Kit is the first off-the-shelf kit for generation of hepatic spheroids in your own lab.

The Gibco Hepatic Spheroid Kit is the first off-the-shelf kit for generation of hepatic spheroids in your own lab.

  • Maintain cells in culture longer—up to 21 days, >4X longer than cells last in monolayer culture
  • Design your own schedule—cells are ready for plating when you are
  • Interrogate pathways of interest—applicable for toxicity, metabolism, or disease modeling

What you get:

  • Spheroid-qualified hepatocytes
  • Hepatocyte Thaw Medium
  • Plating supplement (Cocktail A + Dexamethasone)
  • Maintenance supplement (Cocktail B + Dexamethasone)
  • Fetal bovine serum
  • Williams' E Medium, no phenol red
  • Nunclon Sphera 96-well U-bottom plates

Please note that components are shipped separately due to differences in storage temperatures.

Simplified protocol

Cryopreserved cells can be thawed and plated at your convenience, in as little sa 60 minutes. Try it on your own, or request a demo with an experienced scientist.

Stringent quality control

Our high standards help ensure only the best spheroid-forming lots become available, maximizing your chance of success. In addition, each lot is tested for:

  • Albumin production in monolayer and spheroid culture
  • Phase I enzyme induction
  • Bile duct formation (CFDA staining)
  • Proper morphology
  • Viability >75%
  • Attachment efficiency
  • Monolayer formation and integrity

Important! For best results, do not attempt hepatic spheroid formation in low attachment plates from other sources. Hepatocyte lots are qualified in Nunclon Sphera plates only and use of other plates can lead to variability in spheroid formation.

Flexible readouts

Analyze single spheroids, or pool several for detection in assays such as gene expression, immunostaining, viability, or CYP activity.

Specifications

Content And Storage

• Spheroid-qualified hepatocytes (1 mL, liquid N2 vapor phase only)
• Hepatocyte thaw medium (45 mL, 2°C to 8°C)
• Plating supplement (Cocktail A + Dexamethasone) (18 mL, 2°C to 8°C)
• Maintenance supplement (Cocktail B + Dexamethasone) (20 mL, 2°C to 8°C)
• Fetal bovine serum (2 x 25 mL, -5°C to -30°C)
• Williams' E Medium, no phenol red (2 x 500 mL, 2°C to 8°C. Protect from light.)
• 8 Nunclon Sphera 96-well U-bottom plates
Cell Type Hepatocytes
Form Cryopreserved
Species Human
Donor Source Single Donor
Product Type Hepatocytes
Quantity 1 Kit
Shipping Condition Cells: Dry Shipper, Plates: Room Temperature, Media/Reagents: Dry Ice

Frequently Asked Questions (FAQs)

Who should I contact if I am looking for hepatocytes with particular characteristics?

Our hepatocytes are prequalified and sold according to application, such as induction, short-term metabolism, and transporter uptake. If you require hepatocytes with particular P450 values or donor specifications, contact our Hepatic Biology Tech Support Team (hepaticproducts@thermofisher.com.com) or (866) 952-3559 to help with specific lot recommendations.
Who can I call if I have technical questions about hepatocyte cell culture?

Thermo Fisher Scientific has a dedicated team to answer questions regarding hepatocyte cell culture. You can send a question by email (hepaticproducts@thermofisher.com) or contact us by phone: US toll-free: (866) 952-3559

Are there guidelines for the procurement of human liver tissue?

Thermofisher Scientific works with a variety of human tissue sources, including Tissue and Organ Procurement Organizations, qualified Research Tissue Organizations and prominent academic and medical centers through collaborations that follow rigorous regulations, certifications and/or accreditations (“Source Facilities”). Tissues obtained through source facilities are consistent with the applicable legal and ethical practices of the United States.

Source Facilities are required by federal law to maintain accurate records and donor confidentiality as well as adhere to certain applicable laws governing tissue used for research globally. These include, but are not limited to:

- 45 CFR Part 46, subparts A, B, C, D where applicable (US federal guidance on the use of human subjects in research)
- Health Insurance Portability and Accountability Act (HIPAA) (addressing issues of confidentiality of personal information)
- National Organ Transplant Act (42 CFR Part 482)
- The Uniform Anatomical Gift Act (UAGA) of 1968, revised 1984
Where do you get your human liver tissue?

The human liver cells are derived from tissue obtained from accredited institutions. Consent is obtained by these institutions from the donor or the donor’s legal next of kin, for the use of the tissue and its derivatives for research purpose.
What ratio of 3D-spheroid hepatocytes to hepatic stellate cells should I use in 3D-co-culture?

We have used a ratio between 10:1 and 3:1 for 3D-spheroid hepatocytes to stellate cells for a successful 3D co-culture. We would recommend testing different ratios as the cells may vary from one type of model to another.
Can I co-culture hepatic stellate cells along with 3D-spheroid hepatocytes in 3D culture?

Yes, we have done co-culturing of hepatic stellate cells along with 3D-spheroid hepatocytes.
Can I test the metabolism of my low-turnover compounds using hepatic 3D-spheroids?

We are in the process of testing the metabolism of multiple low-turnover compounds using hepatic spheroids. We have found that a single spheroid containing 3,000 cells can be used for metabolic studies of high-turnover compounds.
How long are the hepatic 3D-spheroids functionally viable?

We have found that hepatic 3D spheroids can be functionally viable for up to 28 days.
How do I change the media for hepatic 3D-spheroids?

We recommend changing 50% of the media every 48-72 hr. You can either discard the media very carefully, using a multi-channel pipette, without disturbing the spheroids, or a programmable plate washer can be used.
What are Cryopreserved 3D-Spheroid Qualified Human Hepatocytes? Why is it that every batch of human hepatocytes does not form 3D-spheroids?

Cryopreserved 3D-Spheroid Qualified Human Hepatocytes are primary hepatocytes isolated from a single donor that can form 3D-spheroids using Nunclon Sphera Plates within 5 days of seeding. We are not sure why every batch of human hepatocytes does not form 3D-spheroids. We speculate that this is a result of donor-to-donor variability and variation in the quality of tissue.

For Research Use Only. Not for use in diagnostic procedures.