Applied Biosystems™ Glass Syringe, 1.0 mL

Polymer in the syringe should be changed every three days. Do not return unused polymer to the bottle.

Buffer and water should be changed every 48 hours or, if the run lasts for more than 48 hours, after the run is complete.

The pump block needs to be cleaned when:

Installing a syringe.

Switching applications (i.e., from sequencing to fragment analysis).

Shutting down the instrument for a long period of time.

If the instrument has run for more than 4 days without any of the above occurring.

Appearance of unusual spikes in the electropherogram.

If the syringe containing the polymer has been on the instrument for more than a week, it is important to clean the syringe and the block, and to change the polymer. At room temperature, enough urea decomposition can occur in one week to cause transient current increases during electrophoreses. If polymer is allowed to dry in the pump block channel, the pump block may be ruined.

A message will appear at the start of the run telling you if you have enough polymer to complete the run. If you should continue without adding more polymer, then the run will be cancelled at the injection where it ran out and an entry will be made into the run log that there is no polymer. The following injections will appear to run for 1–2 minutes each and will be recorded into the run log. No data will be collected for these injections.