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Gibco™ Viral Production Cells DFS Item

Gibco Viral Production Cells are a derivative of the HEK 293F cell line and are a core component of the LV-MAX Lentiviral Production System (Cat. No. A35684).

Supplier:  Gibco™ A35827

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Catalog No. A35827

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This chemical may require us to obtain additional information for our regulatory and chemical compliance records. If required, we will contact you for this information once your order is placed.


Description

Description

Gibco Viral Production Cells are a derivative of the HEK 293F cell line and are a core component of the LV-MAX Lentiviral Production System (A35684). Viral Production Cells have been adapted to a special chemically-defined, serum-free and protein-free LV-MAX Production Medium (A3583401). They are maintained in suspension culture and will grow to high density in LV-MAX Production Medium. Viral Production Cells are highly transfectable by LV-MAX transfection reagent (A35348) and generate superior lentiviral production compared to polyethyleneimine (PEI)-based lentiviral production methods.

  • Suspension culture cells facilitate high-throughput screening or scale-up
  • Higher viral production levels than other suspension HEK 293 cells
  • Rapid cell growth and high culture viabilities

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in LV-MAX Production Medium and 10% DMSO. The cells should be thawed directly into LV-MAX Production Medium.

The cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable in volume from 96-deep well blocks to 2-L flasks and bioreactors. Lentiviral vector harvest is typically performed at two days post-transfection.

Greener Choice Claims
  • Environmental benefits include:
    • Recycled Content
    • Renewable Energy
Specifications

Specifications

Store in liquid nitrogen until ready to use. Do not store the cells at -80°C.
Suspension Cell Culture
6 x 1 mL
Viral Production Cell-Line
Viral Production Cells
Dry Ice
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For Research Use Only. Not for use in diagnostic procedures.