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Gibco™ Viral Production Cells

Catalog No. A35827
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Quantity:
1 mL
6 x 1 mL
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Catalog No. Quantity
A35827 6 x 1 mL
A35347 1 mL
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Catalog No. A35827 Supplier Gibco™ Supplier No. A35827

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Viral Production Cells are a derivative of the HEK 293F cell line and are a core component of the LV-MAX Lentiviral Production System.

Gibco Viral Production Cells are a derivative of the HEK 293F cell line and are a core component of the LV-MAX Lentiviral Production System (Cat. No. A35684). Viral Production Cells have been adapted to a special chemically-defined, serum-free and protein-free LV-MAX Production Medium (A3583401). They are maintained in suspension culture and will grow to high density in LV-MAX Production Medium. Viral Production Cells are highly transfectable by LV-MAX Transfection reagent (A35348) and generate superior lentiviral production compared to polyethyleneimine (PEI)-based lentiviral production methods.

  • Suspension culture cells facilitate high-throughput screening or scale-up
  • Higher viral production levels than other suspension HEK 293 cells
  • Rapid cell growth and high culture viabilities

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in LV-MAX Production Medium and 10% DMSO. The cells should be thawed directly into LV-MAX Production Medium.

The cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable in volume from 96-deep well blocks to 2-L flasks and bioreactors. Lentiviral vector harvest is typically performed at two days post-transfection.

Specifications

Content And Storage Store in liquid nitrogen until ready to use. Do not store the cells at -80°C.
Cell Line Viral Production Cell-Line
Culture Type Suspension Cell Culture
Product Type Viral Production Cells
Quantity 6 x 1 mL
Shipping Condition Dry Ice

For Research Use Only. Not for use in diagnostic procedures.