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  7. Gibco™ Sf-900™ II SFM

Gibco™ Sf-900™ II SFM

Catalog No. 10902187
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Catalog No. 10902187 Supplier Gibco™ Supplier No. 10902187
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Description

Optimized for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS)

Sf-900 II SFM is a serum-free, protein-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells. This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines.

  • Superior long-term, high-density growth
  • Optimized for recombinant protein production
  • Serum-free, protein-free, ready-to-use formulation
  • Scalable in bioreactors

Bioproduction, Cell Culture, Insect Cell Culture, Sf9 and Sf21 Cell Culture, Viral Vaccine Production

Order Info

Shipping Condition: Room temperature

Specifications

Cell Line Sf21, Sf9
Cell Type Insect Cell
Classification Protein-free, Serum-free
Form Liquid
Product Type Insect Cell Serum Free Medium (SFM)
Serum Level Serum-free
With Additives Glutamine
Product Line Gibco, Sf-900, SynerGy
Quantity 20 L
Shipping Condition Room Temperature
Species S. frugiperda, Spodoptera frugiperda
Content And Storage Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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Frequently Asked Questions (FAQs)

Can Sf9 cells in Sf-900 III SFM (Cat. No. 12659017) be thawed and grown in Sf-900 II SFM instead?

It should be okay to thaw the cells into Sf-900 II SFM. This is a richer media compared to the Sf-900 III SFM so the cells would have an easy time adapting. We would recommend taking the cells through 3 passages in the new medium before using them for any experiments as that they have enough time to adapt.

What antimicrobials can be used in insect culture and at what concentration?

Many antibiotics are suitable for use with insect cells. The following antibiotics are commonly used:
- Penicillin/Streptomycine: 50-100 U/mL; 50-100 µg/mL
- Amphotericin B (Fungizone antimycotic): 0.25 µg/mL
- Gentamicin: 0.5 mL of 10 mg/mL solution in 500 mL media (final concentration: 10 µg/mL)

Is it necessary to heat-inactivate my serum before adding it to my medium?

Heat inactivation is not necessary. Our team has routinely used serum that has not been heat-inactivated, and we have not observed any effect on cell growth or morphology.

Many cells do not require heat-inactivated FBS. Some cells prefer heat-inactivated FBS. For instance, we use heat-inactivated FBS for our insect cell lines, i.e., Sf9 and Sf21 cells.

Do you offer serum-free insect media?

Yes, we offer a variety of serum-free insect media. Please go here (http://www.thermofisher.com/us/en/home/life-science/cell-culture/insect-cell-culture/insect-cell-culture-misc/serum-free-media.html?icid=cvc-insect-media-c2t2) to view the media we offer and the differences between them.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.