Please login to your online account to display your discounted pricing

Gibco™ RPMI 1640 Medium, No Glutamine DFS Item

Originally developed to culture human leukemic cells in suspension and as a monolayer

$39.54 - $354.90

Specifications

Format Liquid
Formulation Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt, L-Valine, Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4-7H2O), Thiamine hydrochloride, Vitamin B12, i-Inositol
Packaging Sustainable packaging
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
View More Specs
Requires:  Supplementation

 Disclaimers

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For in vitro diagnostic use.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

21870076

DFS Item
 
life technologies
21870076
500mL Each for $39.54

21870084

DFS Item
 
life technologies
21870084
1000mL Each for $62.74

21870100

DFS Item
 
life technologies
21870100
6 x 1000mL Each for $354.90

21870092

DFS Item
 
life technologies
21870092
10 x 500mL Each for $333.90
Description & Specifications

Specifications

Format Liquid
Formulation Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt, L-Valine, Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4-7H2O), Thiamine hydrochloride, Vitamin B12, i-Inositol
Packaging Sustainable packaging
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture

Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

  • RPMI is modified as follows: with phenol red; without HEPES, L-glutamine
  • RPMI 1640 Medium is unique from other media because it contains reducing agent glutathione and high concentrations of vitamins
  • Contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • Vitamins inositol and choline are present in high concentrations
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

Shipping Condition: Room Temperature

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.