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Gibco™ RPMI 1640 Medium, GlutaMAX™ Liquid Supplement, HEPES DFS Item

Originally developed to culture human leukemic cells in suspension and as a monolayer

$334.95 - $494.55

Specifications

Format Liquid
Formulation Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, HEPES, L-Alanyl-Glutamine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine, L-Glutamic Acid, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Thiamine hydrochloride, Vitamin B12, i-Inositol
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
View More Specs
Requires:  Supplementation

 Disclaimers

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF).

Products
Catalog Number Mfr. No. Quantity Price Quantity    

72400153

DFS Item
 
life technologies
72400153
10L Each for $494.55

72400146

DFS Item
 
life technologies
72400146
5L Each for $334.95
Description & Specifications

Specifications

Format Liquid
Formulation Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, HEPES, L-Alanyl-Glutamine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine, L-Glutamic Acid, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Thiamine hydrochloride, Vitamin B12, i-Inositol
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

  • Modified as follows: with GlutaMAX™, phenol red, HEPES
  • Contains reducing agent glutathione and high concentrations of vitamins
  • Contains biotin, vitamin B12 and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • Vitamins inositol and choline are present in very high concentrations
  • GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture

Shipping Condition: Room Temperature

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