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Gibco™ RPMI 1640 Medium DFS Item

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

$453.00 - $736.00

Specifications

Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Line HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell Type Leukemic Cells
Classification Animal Origin-free
Concentration 1 X
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
11875168 DFS Item
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Gibco™
11875168
5 L
Each for $453.00
 
11875176 DFS Item
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Gibco™
11875176
10 L
Each for $736.00
 
Description

Description

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES

The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Leukemic Cells
1 X
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sterile-filtered
No HEPES, No Sodium Pyruvate
Gibco™, SynerGy™
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Animal Origin-free
Liquid
Standard Serum Supplementation
Glutamine, Phenol Red
cGMP-compliant under the ISO 13485 standard
Room Temperature
SDS
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.