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Gibco™ Recovery™ Cell Culture Freezing Medium DFS Item

Complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3

Manufacturer:  Gibco™ 12648010

Catalog No. 12648010



For many years, researchers have used a common recipe for making freezing medium: High-glucose Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% serum and 10% DMSO. Recovery Cell Culture Freezing Medium is an optimized version of this classic formulation, using Gibco Bovine Serum and Fetal Bovine Serum to provide improved cell recovery and viability after thawing.

  • Suitable for a wide variety of mammalian cells
  • Can be used to freeze most mammalian cells cultured in DMEM, DMEM/F-12, MEM, RPMI 1640, and Ham's F-12
  • Quality tested for pH, osmolality, sterility, and endotoxin
  • Each lot is performance tested using CHO-K1 cells

Cell Counting, Viability & Cryopreservation, Cell Culture, Mammalian Cell Culture, Gene Expression Analysis & Genotyping, In Vitro Transcription, PCR & Real-Time PCR

Order Info

Shipped on dry ice.


Manufactured at a cGMP-compliant facility registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards.



Cell Culture Freezing Medium
Research Grade
Gibco™, Recovery™
Phenol Red
DMSO (10%)
Mammalian Cell Culture
Standard Serum
Dry Ice
12 Months
Phenol Red
High Glucose
Product Certifications


For Research Use Only. Not for use in diagnostic procedures.