Opti-MEM is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts.
- Opti-MEM is modified as follows: with L-glutamine, phenol red
- Unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements
- These additional components allow for a reduction in serum supplementation by at least 50%
- Uses sodium bicarbonate buffer system (2.4g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation
Cell Culture, Mammalian Cell Culture, Plasmid Transfection, RNAi Transfection, Synthetic siRNA Transfection, Transfection, shRNA and miR RNAi Plasmid Transfection
Shipping Condition: Room Temperature
|Storage: 2-8° C. Shelf life: 30 months from date of manufacture|