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Gibco™ MEM (Temin's modification) (2X), no phenol red DFS Item

Modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media

Manufacturer:  Gibco™ LS11935046

Catalog No. 11935046



Temin's Modified Eagle Medium (MEM) can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes.

  • Modified as follows: with L-glutamine, Earle's salts, sodium bicarbonate; without HEPES, phenol red
  • Temin's Modification is 2x MEM with 4x vitamins and amino acids of original MEM
  • Formulation contains Earle's salts for use in a CO2 incubator
  • Made with Earle's salts
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (8.4g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature


Gibco MEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.



Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Room Temperature
500 ml
High Glucose
No Sodium Pyruvate
No Phenol Red

For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.