McCoy's 5A (modified) Medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues. This McCoy's 5A is modified as follows: with high glucose, L-glutamine, bacto-peptone, phenol red; without sodium pyruvate, HEPES.
- Modification of Basal Medium 5A
- Contains reducing agent glutathione, bacto-peptone, and a high level of glucose
- Includes Dr. Hsu's addition of Hank's salts to enable use outside CO2 incubator
- Requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (2.2g/L), and therefore requires 5-10% CO2 environment to maintain physiological pH
- Sustainable packaging
Cell Culture, Mammalian Cell Culture
Shipping Condition: Room Temperature
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|Ascorbic Acid, Bacto-Peptone, Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, L-Alanine, L-Arginine hydrochloride, L-Asparagine, L-Aspartic acid, L-Cysteine, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Nicotinic acid (Niacin), Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxal hydrochloride, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, Vitamin B12, i-Inositol|
|Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture|