missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Gibco™ Mccoy's 5A (Modified) Medium DFS Item

Supports propagation of many types of primary cells, established cell lines, and explants from biopsy tissues

$54.50 - $452.00

Specifications

Format Liquid
Formulation Ascorbic Acid, Bacto-Peptone, Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, L-Alanine, L-Arginine hydrochloride, L-Asparagine, L-Aspartic acid, L-Cysteine, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Nicotinic acid (Niacin), Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxal hydrochloride, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, Vitamin B12, i-Inositol
Packaging Sustainable packaging
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
View More Specs

Requires: Supplementation



Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
16600082 Life Technologies
LS16600082
500mL Each for $54.50
16600108 Life Technologies
LS16600108
10 x 500mL Each for $452.00
Specifications

Description

McCoy's 5A (modified) Medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues. This McCoy's 5A is modified as follows: with high glucose, L-glutamine, bacto-peptone, phenol red; without sodium pyruvate, HEPES.

  • Modification of Basal Medium 5A
  • Contains reducing agent glutathione, bacto-peptone, and a high level of glucose
  • Includes Dr. Hsu's addition of Hank's salts to enable use outside CO2 incubator
  • Requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.2g/L), and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Description & Specifications

Specifications

Liquid
Ascorbic Acid, Bacto-Peptone, Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, L-Alanine, L-Arginine hydrochloride, L-Asparagine, L-Aspartic acid, L-Cysteine, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Nicotinic acid (Niacin), Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxal hydrochloride, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, Vitamin B12, i-Inositol
Sustainable packaging
Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture