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Gibco™ McCoy's 5A (Modified) Medium
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Catalog No. 16600108
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500 mL
10 x 500 mL
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16600108 10 x 500 mL
16600082 500 mL
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Catalog No. 16600108 Supplier Gibco™ Supplier No. 16600108
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Description

Requires

Supplementation

McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.

McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

This McCoy's 5A is modified as follows:

With: High Glucose, L-glutamine, Bacto-peptone, Phenol Red

Without: Sodium Pyruvate, HEPES

The complete formulation is available.

Using McCoy's 5A (modified) Medium

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator. McCoy's 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy's 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

Cell Line Rat fibroblasts
Cell Type Biopsy Tissues
Classification Animal Origin
Concentration 1 X
Form Liquid
Product Type McCoy's 5A Modified Medium
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose, Glutamine, Phenol Red, Bacto-peptone
Without Additives No HEPES, No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 10 x 500 mL
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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