Gibco™ LV-MAX™ Lentiviral Production System 

Description

The Gibco LV-MAX Lentiviral Production System is the first optimized system that provides a scalable and high-yield lentiviral vector production platform. It is based on a suspension, high-density culture of HEK 293-derived Viral Production Cells adapted to a special chemically defined, serum-free and protein-free LV-MAX Production Medium. Optimal viral titer yield is mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel lentiviral-specific enhancer and supplement. All components work synergistically to generate superior and functional lentiviral particles under serum-free conditions compared to conventional polyethylenimine (PEI).

The LV-MAX Lentiviral Production System provides:

• High titer—greater than 1 x 10⁸ TU/mL (unconcentrated LVVs-GFP)
• Scalable suspension system—from 96 deep-well plate to 2-L bioreactor
• Serum-free—eliminating animal-origin components and associated risks
• Easy, robust culture and transfection protocols—fits into current transient production workflows

The components included in the LV-MAX Lentiviral Production System are: two vials of frozen Viral Production Cells, one liter of LV-MAX Production Medium, and one LV-MAX Transfection Kit sufficient to produce 0.3 L of lentivirus. All components of the LV-MAX Lentiviral Production System are also available for purchase separately. Also required, but not included in this kit, is Gibco Opti-MEM I Reduced Serum Medium (Cat. No. 31985062).