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Gibco™ Ham's F-12K (Kaighn's) Medium DFS Item

A modification of Ham's F-12 Nutrient Mixture

$65.00 - $568.00


Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
21127022 View Documents Gibco™
500mL Each for $65.00
21127030 View Documents Gibco™
10 x 500mL Each for $568.00


Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment. This F-12K (Kaighn's) Medium is modified as follows: with L-glutamine, phenol red; without HEPES.

  • Contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate
  • Additions allow medium to be supplemented with very low levels of serum or defined components, for some cell types
  • Contains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS)
  • FBS concentration must be optimized for each cell line
  • Uses sodium bicarbonate buffer system (2.5g/L), and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging


Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature




For Research Use Only. Not for use in diagnostic procedures.