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Gibco™ Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement DFS Item

Designed for serum-free single-cell plating of Chinese hamster ovary cells

$55.65 - $527.10

Specifications

Format Liquid
Formulation Biotin, Calcium Chloride (CaCl2-2H2O), Choline chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate, D-Glucose (Dextrose), Ferric sulfate (FeSO4-7H2O), Folic Acid, Glycine, Hypoxanthine, L-Alanine, L-Alanyl-L-Glutamine, L-Arginine hydrochloride, L-Asparagine (freebase), L-Aspartic acid, L-Cysteine hydrochloride, L-Glutamic Acid, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Linoleic Acid, Lipoic Acid, Magnesium Chloride (MgCl2-6H2O), Niacinamide, Phenol Red, Potassium Chloride (KCl), Putrescine 2HCl, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Pyruvate, Thiamine hydrochloride, Thymidine, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
Packaging Sustainable packaging
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
View More Specs
Requires:  Supplementation

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

31765035

DFS Item
 
life technologies
31765035
500mL Each for $55.65

31765092

DFS Item
 
life technologies
31765092
10 x 500mL Each for $527.10
Description & Specifications

Specifications

Format Liquid
Formulation Biotin, Calcium Chloride (CaCl2-2H2O), Choline chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate, D-Glucose (Dextrose), Ferric sulfate (FeSO4-7H2O), Folic Acid, Glycine, Hypoxanthine, L-Alanine, L-Alanyl-L-Glutamine, L-Arginine hydrochloride, L-Asparagine (freebase), L-Aspartic acid, L-Cysteine hydrochloride, L-Glutamic Acid, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Linoleic Acid, Lipoic Acid, Magnesium Chloride (MgCl2-6H2O), Niacinamide, Phenol Red, Potassium Chloride (KCl), Putrescine 2HCl, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Pyruvate, Thiamine hydrochloride, Thymidine, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
Packaging Sustainable packaging
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture

Ham's F-12 Nutrient Mixture (F-12) has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells.

  • Contains zinc, putrescine, hypoxanthine, and thymidine
  • Serum-free growth of CHO cells in F-12 has led to variety of improved formulations
  • It is modified as follows: with GlutaMAX™, phenol red. Gibco F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in easy-to-use format
  • Contains no proteins or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (1.176g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

Shipping Condition: Room Temperature

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