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  7. Gibco™ Ham's F-12 Nutrient Mix

Gibco™ Ham's F-12 Nutrient Mix
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Catalog No. 11765070
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11765070 6 x 1000 mL
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Catalog No. 11765070 Supplier Gibco™ Supplier No. 11765070
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Description

Requires

Supplementation

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells.

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:

With: L-glutamine, Phenol Red

The complete formulation is available.

Using F-12

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

Cell Line CHO, COS-7, and rat prostate epithelial cells
Cell Type Primary Rat Astrocytes
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type Ham's F-12 Nutrient Mixture
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Glutamine, Phenol Red, Sodium Pyruvate
Without Additives No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 6 x 1000 mL
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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