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Gibco™ Grace's Insect Medium, unsupplemented
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Catalog No. 11595030
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11595030 500 mL
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Catalog No. 11595030 Supplier Gibco™ Supplier No. 11595030
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Description

Useful during transfection of insect cells or for protein labeling studies with methionine

Designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. One supplemented, widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). Life Technologies offers a variety of Gibco Media options for a range of insect cell culture applications. The complete formulation is available.It does not contain lactalbumin hydrolysate or yeastolate, commonly found in the supplemented version. Gibco Grace's Unsupplemented Insect Medium is also made without methionine for protein labeling studies.

  • Manufactured at cGMP compliant facility located in Grand Island, New York
  • The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards
  • Requires supplementation with 10% (FBS), 50 mg/L methionine, 3.33 g/L lactalbumin hydrolysate and 3.33 g/L yeastolate before use as a growth medium

Cell Culture, Insect Cell Culture, Sf9 and Sf21 Cell Culture

Order Info

Shipping Condition: Room temperature

Specifications

Cell Line Sf21, Sf9
Cell Type Insect Cell
Form Liquid
Product Type Grace's Insect Medium
With Additives Glutamine, Sodium Bicarbonate
Without Additives No Lactalbumin Hydrolysate, No Yeastolate, No Methionine
Product Line Gibco
Quantity 500 mL
Shipping Condition Room Temperature
Inorganic Salts Calcium, Magnesium
Content And Storage Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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Frequently Asked Questions (FAQs)

For how long can I store Grace's Insect Medium, unsupplemented at room temperature?

We ship this product under “ambient” conditions throughout the year. We have performed shipping studies that shown components to be stable for at least 7 days at ambient temperatures. The important factor is to ensure that the media is not exposed to light at the warmer temperatures as there are vitamins and amino acids that will breakdown with light exposure.
What additional supplements are required in Grace's Insect Cell Culture Medium, Unsupplemented to make a "complete" formulation that will support insect cell growth?

Grace's Insect Cell Culture Medium, Unsupplemented requires addition of 3.33 g/L of lactalbumin hydrolysate and 3.33 g/L of yeastolate for the “base” growth medium. For the “complete” growth medium that will support insect cell growth, 10% fetal bovine serum must also be added. This “complete” growth medium is also referred to as TNM-FH.

Do you offer a methionine-free media for baculovirus expression?

Yes, we offer our methionine-free Grace's Insect Medium (Cat. No. 11595030).

For Research Use Only. Not for use in diagnostic procedures.