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Gibco™ Puromycin Dihydrochloride
Description
Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene.
- Used in cell biology as selection antibiotic in mammalian cell culture systems
- Recommended working concentration ranges from 0.2–5.0μg/mL
- Can be toxic to eukaryotic cells at concentrations as low as 1μg/mL
- Supplied at 10mg/mL in 20 mM HEPES buffer (pH 7.2–7.5)
Cell Culture, Cloning, Drosophila S2 Cell Culture, Insect Cell Culture, Mammalian Cell Culture, Selection, Sf9 & Sf21 Cell Culture, Transfection, Transformation
Order Info
Shipping Condition: Dry Ice
Specifications
Specifications
| Content And Storage | Storage conditions: -5 to -20°C Shipping conditions: Frozen Shelf life: 12 months from date of manufacture |
| Cell Type | Eukaryotic Cells, Prokaryotic Cells |
| Concentration | 10 mg/mL |
| Culture Type | Mammalian Cell Culture, Insect Cell Culture |
| Form | Liquid |
| Product Type | Antibiotic |
| Sterility | Sterile-filtered |
| With Additives | HEPES |
| For Use With (Application) | Eukaryotic Selection⁄Stable Cell Line Generation |
| Product Line | Gibco |
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For Research Use Only. Not for use in diagnostic procedures.