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Gibco™ DMEM, powder, low glucose, pyruvate

Catalog No. 31600091
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Catalog No. 31600091 Supplier Gibco™ Supplier No. 31600091
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Description

Requires

Supplementation

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With: Low Glucose, L-glutamine, Sodium Pyruvate, Phenol Red

Without: Sodium Bicarbonate, HEPES

The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Please note that the shelf life on delivered products varies. This product has a minimum shelf life of 6 months at time of shipment. For more information regarding product shelf life, including details on specific product lots, please contact Customer Service.

Order Info

Shipping Condition: Room Temperature

Specifications

Cell Line HeLa, 293, Cos-7, and PC-12
Cell Type Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Form Powder
Serum Level Standard Serum Supplementation
With Additives Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Without Additives No HEPES, No Sodium Bicarbonate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 50 L
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
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Frequently Asked Questions (FAQs)

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

What is the shelf life of my powdered media once I reconstitute it?

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.