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Gibco™ DMEM, high glucose, HEPES, no phenol red DFS Item

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

$138.00 - $1148.00

Specifications

Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Line HeLa, 293, Cos-7, and PC-12
Cell Type Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Classification Animal Origin-free
Concentration 1 X
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
21063029 DFS Item
View Documents Promotion Details
Gibco™
21063029
500 mL
Each for $138.00
 
21063045 DFS Item
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Gibco™
21063045
10 x 500 mL
Each for $1,148.00
 
Description

Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
With: High Glucose, L-glutamine, HEPES
Without: Sodium Pyruvate, Phenol Red

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Compliance

DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

Specifications

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
1 X
DMEM (Dulbecco's Modified Eagle Medium)
Sterile-filtered
No Phenol Red, No Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
Room Temperature
HeLa, 293, Cos-7, and PC-12
Animal Origin-free
Liquid
Standard Serum Supplementation
High Glucose, Glutamine, HEPES
Sustainable packaging
Gibco™
SDS
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.