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Gibco™ DMEM, high glucose, HEPES 
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Supplier: Gibco™ 12430054
Description
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With: High Glucose, L-glutamine, HEPES, Phenol Red
Without: Sodium Pyruvate
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Greener Choice Claims
- Environmental benefits include:
- Recyclable
Specifications
| HeLa, 293, Cos-7, and PC-12 | |
| Animal Origin-free | |
| Liquid | |
| Standard Serum Supplementation | |
| Sterile-filtered | |
| No Sodium Pyruvate | |
| 4500 mg/L | |
| Gibco™ | |
| Room Temperature |
| Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells | |
| 1 X | |
| DMEM (Dulbecco's Modified Eagle Medium) | |
| Sterile-filtered | |
| High Glucose, Glutamine, HEPES, Phenol Red | |
| Mammalian cell culture | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL | |
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.