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Gibco™ DMEM/F-12, HEPES DFS Item

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEMF/12, HEPES is used in cell culture processing applications.

$70.25 - $568.00

Specifications

Format Liquid
Formulation Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Ferric sulfate (FeSO4-7H2O), Folic Acid, Glycine, HEPES, Hypoxanthine Na, L-Alanine, L-Arginine hydrochloride, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Linoleic Acid, Lipoic Acid, Magnesium Chloride (anhydrous), Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Putrescine 2HCl, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Thiamine hydrochloride, Thymidine, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
Packaging Sustainable packaging
Storage Requirements Storage: 2-8° C. Protect from light
Product Type DMEMF/12 media
View More Specs


Products ${productFamilyLength}
Catalog Number Mfr. No. For Use With (Application) Quantity Price Quantity  
Catalog Number Mfr. No. For Use With (Application) Quantity Price Quantity  
11330057 Gibco™
LS11330057
Mammalian cell culture 10 x 500mL Each for $568.00
11330032 Gibco™
LS11330032
Mammalian cell culture 500mL Each for $70.25
11330099 GIbco™
LS11330099
Antibody and cell culture production, Mammalian cell culture 5L Each for $327.00
11330107 Gibco™
LS11330107
Antibody and cell culture production, Mammalian cell culture 10L Each for $510.00
Specifications

Description

  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

Order Info

Shipping Condition: Room Temperature

Description & Specifications

Specifications

Liquid
Sustainable packaging
DMEMF/12 media
Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Ferric sulfate (FeSO4-7H2O), Folic Acid, Glycine, HEPES, Hypoxanthine Na, L-Alanine, L-Arginine hydrochloride, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Linoleic Acid, Lipoic Acid, Magnesium Chloride (anhydrous), Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Putrescine 2HCl, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Thiamine hydrochloride, Thymidine, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
Storage: 2-8° C. Protect from light
L-Glutamine, Phenol Red, HEPES