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Gibco™ CD Hybridoma Medium
Description
CD Hybridoma Medium is an animal origin-free, chemically defined, protein-free medium.
- Ability to support suspension and stationary hybridomas and myelomas
- Formulated without L-glutamine for stability
- Animal origin-free, protein-free, and chemically defined formulation
Ability to support suspension and stationary hybridomas and myelomas
- Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco CD Hybridoma Medium provides high cell densities and IgG production
- Cholesterol-dependent cultures such as NS0 require the addition of 250X Cholesterol Lipid Concentrate
Formulated without L-glutamine for stability
- Gibco CD Hybridoma Medium requires the addition of 8 mM L-glutamine or GlutaMAX™ supplement before use
- GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
Animal origin-free, protein-free, and chemically defined formulation
- Gibco CD Hybridoma Medium is animal origin-free, protein-free, and chemically defined, allowing for easier purification of your protein of interest
- Gibco chemically defined media contain no proteins, hydrolysates, or components of unknown composition
Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Cell Type | Myeloma Cells, Hybridomas |
| Classification | Animal Origin-free, Chemically-defined, Protein-free |
| Form | Liquid |
| Product Type | Hybridoma Medium |
| Without Additives | No Glutamine, No Phenol Red |
| Product Line | Gibco |
| Quantity | 1000 mL |
| Shipping Condition | Room Temperature |
| Content And Storage | Storage conditions: 2-8°C. Protect from light Shipping conditions: Ambient Shelf life: 18 months from date of manufacture |
Frequently Asked Questions (FAQs)
Cells can be adapted by Sequential or Direct Adaptation. Suggested protocols for each are below, and you can also find more information by searching "Adaptation of Cell Cultures to a Serum-Free Medium" from our website home page.
SEQUENTIAL ADAPTATION
1) Subculture the cells growing in serum-supplemented medium into a 25%:75% mixture of SFM and serum supplemented medium.
2) When the cell density is 5 x 10E5 cells/ml, subculture the cells into a 50%:50% mixture of SFM and serum supplemented medium at a cell density 2.5 x 10E5 to 3 x 10E5 cells/ml.
3) Continue to subculture after the cell density 5 x 10E5 cells/ml in gradually increasing proportions of SFM until the serum is ~0.1% with about 85% cell viability.
4) Subculture the cells into SFM with an innoculum of 2.5 x 10E5 to 3 x 10E5 cells/ml.
5) When the cell density is 1 x 10E6 to 3 x 10E6 cells/ml (4 to 6 days post planting) subculture the cells again.
6) Stock cultures of SFM adapted cells should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.
DIRECT ADAPTATION
Some cells can be directly adapted from serum-containing medium to SFM. For direct adaptation, the cell innoculum should be 1.5 x 10E5 to 3 x 10E5 cells/ml.
Cells should be subcultured when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml. Cells are fully adapted to SFM when the cell density is 2 x 10E6 to 4 x 10E6 cells/ml after 4 to 7 days in culture.
Stock cultures of cells adapted to SFM should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.
Some cells, such as insect cells, are sensitive to changes in their medium. By sequentially adapting cells, the medium is changed with minimal effects on cell growth.
CD Hybridoma Medium can be filtered by 0.2 µm filtration.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.