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Gibco™ Blasticidin S HCl, powder

Catalog No. R21001
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R21001 50 mg
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Catalog No. R21001 Supplier Gibco™ Supplier No. R21001
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Description

Bacterial selective antibiotic in concentration range of 50G–100μg/mL

Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in bacteria and eukaryotes, while also active in fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian and bacterial cells. The recommended working concentration ranges from 1 to 30 μg/mL depending on the cell line and 25–100 μg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines can be generated in less than one week.

  • Blasticidin S acts quickly by inhibiting peptide bond formation by the ribosome
  • Used for prevention of contamination of cell cultures
  • Recommended working concentration ranges from 2 to 2μg/mL depending on the cell line
  • Resistance is conferred by expression of either one of two Blasticidin S deaminase genes: BSD (2) or bsr

Resistance to blasticidin S is conferred by BSR and BSD, isolated from Bacillus cereus K55-S and Aspergillus terreus respectively. The BSR resistance gene encodes blasticidin S deaminase, which catalyzes the conversion of blasticidin S to deaminohydroxyblasticidin S. Deaminohydroxyblasticidin S is a biologically inactive derivative of blasticidin S and does not interact with or inhibit prokaryotic or eukaryotic ribosomes. The BSD resistance gene also encodes a blasticidin S deaminase, which catalyzes a similar reaction to the BSR deaminase.

For bacterial selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0 to maintain the activity of basticidin S. A kill curve is recommended in order to determine the minimum effective blasticidin S concentration to kill non-resistant cells.

Applications: Cell Culture, Cloning, Drosophila S2 Cell Culture, High Five™ Cell Culture, Insect Cell Culture, Mammalian Cell Culture, Selection, Sf9 & Sf21 Cell Culture, Transfection, Transformation

Order Info

Shipping conditions: Ambient

Specifications

Color White, off-white
Content And Storage Storage conditions: -5 to -30°C
Shipping conditions: room temperature
Shelf life: Lot Dependent
Cell Type Eukaryotic Cells, Prokaryotic Cells
Concentration 50 to 100 μg/mL
Culture Type Mammalian Cell Culture, Insect Cell Culture
Form Powder
Product Type Antibiotic
Sterility Sterile
With Additives HEPES
For Use With (Application) Bacterial Selection
Product Line Gibco
Quantity 50 mg
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For Research Use Only. Not for use in diagnostic procedures.