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Gibco™ Basal Medium Eagle (BME)
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Catalog No. 21010046
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500 mL
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21010046 500 mL
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Catalog No. 21010046 Supplier Gibco™ Supplier No. 21010046
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Description

Requires

Supplementation

Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells.

Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.

This BME is manufactured as follows:

  • With: Phenol Red
  • Without: L-glutamine, HEPES

The complete formulation is available.

BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2 incubator.

BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

Cell Line HeLa, mouse fibroblasts, WI-38 and MRC-5
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type Basal Medium Eagle (BME)
Sterility Sterile-filtered
With Additives Low Glucose, Phenol Red
Without Additives No Glutamine, No HEPES, No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 500 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.