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Gibco™ Advanced RPMI 1640 Medium DFS Item

Advanced RPMI (Roswell Park Memorial Institute) 1640 is widely used basal medium that allows culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation

$41.77 - $399.00

Specifications

Format Liquid
Formulation AlbuMAX II, Ammonium Metavanadate, Ascorbic Acid phosphate, Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, Cupric Sulfate, D-Calcium pantothenate, D-Glucose (Dextrose), Ethanolamine, Folic Acid, Glutathione (reduced), Glycine, Human Transferrin (Holo), Insulin Recombinant Full Chain, L-Alanine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Manganous Chloride, Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Pyruvate, Sodium Selenite, Thiamine hydrochloride, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
Packaging Sustainable packaging
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
View More Specs

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.

Requires: Supplementation



Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
12633012 Life Technologies
LS12633012
500mL Each for $41.77
12633020 Life Technologies
LS12633020
10 x 500mL Each for $399.00
Specifications

Description

Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat. This Advanced RPMI 1640 is modified as follows: with glucose, non essential amino acids, sodium pyruvate, phenol red; without L-glutamine, HEPES.

  • Unique from other media due to addition of following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride
  • Requires supplementation with 1-5% Fetal Bovine Serum and 4mM L-glutamine or GlutaMAX™ supplement
  • Many cell lines do not require adaptation to this media
  • FBS concentration must be optimized for each cell line to obtain maximum serum reduction
  • Uses sodium bicarbonate buffer system (3.7g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco Advanced RPMI 1640 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Description & Specifications

Specifications

Liquid
AlbuMAX II, Ammonium Metavanadate, Ascorbic Acid phosphate, Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, Cupric Sulfate, D-Calcium pantothenate, D-Glucose (Dextrose), Ethanolamine, Folic Acid, Glutathione (reduced), Glycine, Human Transferrin (Holo), Insulin Recombinant Full Chain, L-Alanine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Manganous Chloride, Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Pyruvate, Sodium Selenite, Thiamine hydrochloride, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
Sustainable packaging
Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture