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Gibco™ Advanced DMEM
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Numéro de catalogue. p-4919814
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500 mL
10 x 500 mL
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12491015 500 mL
12491023 10 x 500 mL
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Numéro de catalogue. 12491015 Fournisseur Gibco™ Code fournisseur 12491015
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Description

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Supplementation

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.

This Advanced DMEM is manufactured as follows:

With: High Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red

Without: L-glutamine

The complete formulation is available.

Using Advanced DMEM

Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Spécifications

Cell Line MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
Classification Animal Origin
Concentration 1 X
Form Liquid
Product Type DMEM (Dulbecco's Modified Eagle Medium)
Serum Level Reduced Serum
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Without Additives No Glutamine, No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 500 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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For Research Use Only. Not for use in diagnostic procedures.