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Gibco™ 293 H Cells, in CD 293

Catalog No. 11631017
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Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.
Catalog No. 11631017 Supplier Gibco™ Supplier No. 11631017
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Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

Description

Faster growth, good adherence, superior transfection efficiencies and high protein expression levels

Gibco 293-H cells were cloned from the original 293 cell line and adapted to Gibco CD 293 Medium. The 293 cell line is a permanent line established from primary embryonic human kidney and transformed with sheared human adenovirus type 5 DNA. The E1A adenovirus gene is expressed in these cells and participates in transactivation of some viral promoters, allowing these cells to produce very high levels of protein.

  • Quick thaw into suspension culture directly in Gibco CD 293 Medium or Gibco 293 SFM II
  • Faster growth, good adherence, superior transfection efficiencies and high protein expression levels
  • Documented lineage from a low passage Master Cell Bank
  • Tested for quality and performance

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture, Mammalian Expression, Mammalian Expression Cell Lines, Mammalian Protein Production, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Vaccine Production

Order Info

Shipping Condition: Dry Ice

Specifications

Content And Storage 1 vial of 7.5 x 106 cells
Storage conditions: liquid nitrogen (vapor phase)
Shipping conditions: frozen
Cell Line 293H
Species Human
Media Recommendation SFM (Serum-Free Media)
Product Type HEK 293 Cells
Quantity 1.5 mL
Shipping Condition Dry Ice

Frequently Asked Questions (FAQs)

What is the difference between 293, 293H, 293F, and 293FT cells?

The 293 cell line is a permanent line established from primary embryonal human kidney transformed with sheared human adenovirus type 5 DNA (Graham et al., 1977; Harrison et al., 1977). The E1A adenovirus gene is expressed in these cells and participates in transactivation of some viral promoters, allowing these cells to produce very high levels of protein.

The FreeStyle 293-F cell line is a clone of the 293 cell line and is intended for use with the FreeStyle 293 Expression System. These cells are adapted to suspension culture in FreeStyle 293 Expression Medium.

The 293H cell line was also cloned from 293 cells, and like 293F cells can be grown as either suspension or adherent cultures. Neither 293F or 293H attach very well in SFM. In serum-supplemented medium, both attach better, but 293H attaches better than 293F. 293H is also better for transient transfection. 293F cells have a faster doubling time than 293H, and are better suited for selection of stables and large scale cultures.

The 293FT Cell Line is derived from the 293F Cell Line and stably expresses the SV40 large T antigen from the pCMVSPORT6TAg.neo plasmid. Expression of the SV40 large T antigen is controlled by the human cytomegalo-virus (CMV) promoter and is high-level and constitutive.

For Research Use Only. Not for use in diagnostic procedures.