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Thermo Scientific™ GelCode™ Blue Safe Protein Stain
Description
GelCode Blue Safe Protein Stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and membranes. This protein-specific stain allows bands to be viewed directly on a gel or membrane during staining and is compatible with mass spectrometry applications. GelCode Blue Safe Protein Stain is non-corrosive, non-flammable, and is classified as non-hazardous under the U.S. Department of Transportation (DOT) shipping requirements.
GelCode Blue Safe Protein Stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and is formulated to avoid hazardous shipping.
Features include:
- Sensitive—detect down to 9 ng of protein per band in 15 minutes
- Safe—non-corrosive to skin, non-flammable, and safe to ship and store until activated
- Minimal additional reagents—water washes only; no acid-fixative or methanol destain solutions required
- Compatible—suitable for staining 1D and 2D polyacrylamide gels and nitrocellulose and PVDF membranes (multiple protocols provided in the instructions)
- Multiple applications—qualitative visualization, quantitative densitometry, and downstream excision and analysis by mass spectrometry
- Easy to use—add and dissolve activator crystals before first use, then simply add reagent to stain gels
- Stable—store non-activated or activated stain at room temperature for up to one year
Because the supplied activator crystals are packaged and shipped dry in a separate bottle, GelCode Blue Safe Protein Stain is classified as nonhazardous under the U.S. Department of Transportation (DOT) shipping requirements. For proper protein staining, the activator crystals must be dissolved in the staining reagent before use. Add the entire contents of the activator crystals bottle into the GelCode Blue Safe Protein Stain bottle. Dissolve the crystals by mixing end-over-end for one minute, then allow it to remain at room temperature for five minutes before use. Store the activated stain at room temperature.
Specifications
Specifications
| Content And Storage | Store at room temperature. |
| Detection Location | In-Blot Detection, In-Gel Detection |
| Color | Blue |
| Detection Method | Colorimetric |
| Label or Dye | Coomassie |
| Quantity | 3.5 L |
| Target Molecule | Protein |
| Product Line | GelCode |
| Product Type | Protein Stain |
Frequently Asked Questions (FAQs)
Yes, GelCode Blue Safe Stain is compatible with homemade gels and most commercially available pre-cast gels.
Yes. It is compatible with mass spectrometry and 2D electrophoresis, as well as membrane staining.
Yes, you can add up to 20% less crystals and still obtain acceptable staining.
It is possible the GelCode Blue Safe Stain was not activated. When the activator crystals are added, the pH of the stain should be approximately 2. If the stain was not activated, add the crystals and restain the gel; however, if the stain was activated, try increasing the staining time.
The lower level of detection is 9 ng of protein per band.