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Gibco™ FluoroBrite™ DMEM
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Catalog No. a1896702
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A1896701 500 mL
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Description

Gibco™ FluoroBrite™ DMEM features a background fluorescence that is comparable to PBS and 90% lower than that emitted by standard phenol red–free DMEM.

Gibco™ FluoroBrite™ DMEM features a background fluorescence that is comparable to PBS and 90% lower than that emitted by standard phenol red–free DMEM. Formulated to include the required nutrients for routine cell culture when supplemented with 10% fetal bovine serum and 4 mM L-glutamine or GlutaMAX™ Supplement, FluoroBrite™ DMEM is designed to enhance the signal-to-noise ratio of fluorophores, enabling researchers to visualize even the weakest fluorescent events in an environment that promotes optimum cell health. Additional features include:

  • Enhancement of fluorescence signal during live-cell imaging
  • DMEM-based to help preserve cell health

Live-cell fluorescence microscopy is an essential technique for the visualization of fundamentally important and physiologically relevant biological events. A key challenge with this technique is the ability to image weak fluorophores without causing cell damage, photobleaching, or undesirable changes to cell health. FluoroBrite™ DMEM helps address these issues.

Specifications

Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type DMEM (Dulbecco's Modified Eagle Medium)
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
With Additives High Glucose
Without Additives No Glutamine, No Phenol Red, No HEPES, No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Quantity 10 x 500 mL
Content And Storage Store in refrigerator (2–8°C). Protect from light.
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Frequently Asked Questions (FAQs)

Do you offer an alternative or replacement of Invitrogen Live Cell Imaging Solution (Cat. No. A14291DJ)?

An alternative for Live Cell Imaging Solution (LCIS) (Cat. No. A14291DJ) is FluoroBrite DMEM (Cat. Nos. A1896701, A1896702), which, like LCIS, is formulated not to have any fluorescent background or quenchers and is verified for imaging live cells in fluorescence. The product can be found using the link below.

FluoroBrite DMEM (Cat. Nos. A1896701, A1896702)
What is the osmolality of Fluorobrite DMEM?

We do provide osmolality information on the certificate of analysis. All lots of Fluorobrite DMEM (Cat. Nos. A1896701 and A1896702) will meet the osmolality specification of 320-350 mOsm/kg.
I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.
Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

For Research Use Only. Not for use in diagnostic procedures.