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  5. Thermo Scientific™ T7 RNA Polymerase (20 U/μL)

Thermo Scientific™ T7 RNA Polymerase (20 U/μL)

Catalog No. FEREP0111
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Concentration:
20U/µL
Quantity:
5 x 5,000 units
5,000 units
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Catalog No. Concentration Quantity
FEREP0111 20U/µL 5,000 units
FEREP0112 20U/µL 5 x 5,000 units
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Catalog No. FEREP0111 Supplier Thermo Scientific™ Supplier No. EP0111
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Description

Thermo Scientific™ T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.

Thermo Scientific™ Bacteriophage T7 RNA polymerase is DNA-dependent RNA polymerase with strict specificity its respective double-stranded promoter.

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.
  • Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used:for hybridization, in vitro RNA translation; as aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing.

Note:

Consensus promoter sequences: TAATACGACTCACTATA G GGAGA. The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively.

Specifications

Polymerase T7 RNA Polymerase
Quantity 5,000 units
Concentration 20U/µL

Frequently Asked Questions (FAQs)

What is the advantage of using TranscriptAid T7 High Yield Transcription Kit over Thermo Scientific T7 RNA Polymerase with a standard transcription buffer?

TranscriptAid T7 High Yield Transcription Kit is designed to produce both long and short transcripts for applications that require large yields of RNA (milligram amounts). The kit can achieve 10 times greater amount than from conventional in vitro transcription reactions with T7 RNA polymerase due to its unique proprietary enzyme and buffer formulations. Please note that TranscriptAid T7 High Yield Transcription Kit is not recommended for generation of radioactively labelled RNA. Due to large quantities of RNA synthesized with the kit, generation of high specific activity radiolabeled probes would require prohibitively large amounts of radiolabeled nucleotide.
Does the T7 RNA Polymerase (20 U/μl) (Cat. No. EP0111) contain a His-Tag which could be used for purification or conjugation?

The T7 RNA Polymerase (20 U/μl) (Cat. No. EP0111) does not contain a His-Tag, and currently none of our T7 RNA polymerases have a tag available for purification/conjugation.

For Research Use Only. Not for use in diagnostic procedures.