Thermo Scientific™ T7 RNA Polymerase
Catalyze the 5'=>3' synthesis of RNA on either single-stranded or double-stranded DNA with T7 RNA polymerase.
Manufacturer: Thermo Scientific™ EP0113
Bacteriophage T7 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters. It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.
- Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)
Synthesis of unlabeled and labeled RNA that can be used:for hybridization, in vitro RNA translation; as aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing.
Consensus promoter sequences: TAATACGACTCACTATA G GGAGA. The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively.
|5 x 1mL of 5X Transcription Buffer|
|Synthesis of unlabeled and labeled RNA that can be used:for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.|
|T7 RNA Polymerase|